Sense Of Joy, one of the leading fancies for the stanjamesuk.com 1000 Guineas, has been ruled out after soft tissue damage was confirmed on Tuesday.

John Gosden's filly won both her starts as a juvenile but has not been seen since the Prestige Stakes at Goodwood last August.

"We are obviously trying to keep everyone up to speed with the problem," said Teddy Grimthorpe, racing manager to her owner Prince Khalid Abdullah.

"It is not life-threatening but still potentially serious and it will keep her out of the Guineas."

Grimthorpe continued: "The soft tissue problem was confirmed today and I hope it will not be too bad, but we just want to get her properly checked out before we know any more."

Grimthorpe confirmed Fillies' Mile runner-up Proviso would not be called up as a supersub at Newmarket.

"Proviso will not be running in the English 1000 Guineas - she will be running in the Prix Vanteaux."

The Guineas sponsors quickly cut Natagora a point to 4-1 favourite.

Stanjamesuk.com 1000 Guineas, sponsors bet: 4-1 Natagora, 9-2 Muthabara, 11-2 Spacious, 8-1 Cape Amber, 12-1 Kitty Matcham, 14-1 bar.